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I have heard this in a song: 'I can't take my eyes from/off you'. However, I can't remember whether the preposition is from or off. Are both prepositions possible?
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I'm sure the song goes "off-a you " - or maybe it'd a different song!

"off" is the most used, but from IS possible -it has a slightly quaint or achaic ring to it, though.
Comments  
maj, I think both of the prepositions are correct in that context; it means "I can't stop looking at you"Emotion: wink
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If Novalee is a native speaker of the language then he will know for sure whether "from" is accepted in this context

But i would prefer to use "off" than "from". I checked it out with the dictionary
 suzi's reply was promoted to an answer.